Abstract
The authors developed a simple method to measure angiotensin-converting enzyme (ACE) activity using capillary electrophoresis (CE). With hippuryl-L-histidyl-L-leucine (HHL) as the substrate, we separated and quantified the enzymatic product, hippuric acid (HA) using capillary zone electrophoresis (CZE). CZE was performed with a 65 cm fused-silica capillary (55 cm effective length, 75 μm I.D.), using a 10 or 20 mM phosphate solution (pH 6.0) as the running buffer with an applied voltage of 20 kV at room temperature. The HA was identified and monitored at 228 nm.
Recommended Citation
Chiang, M.-C.; Yang, S.-C.; Li, K.-Y.; and Tsai, T.-C.
(2005)
"An assay of angiotensin-converting enzyme activity obtained using capillary electrophoresis,"
Journal of Food and Drug Analysis: Vol. 13
:
Iss.
3
, Article 8.
Available at: https://doi.org/10.38212/2224-6614.2520
