Abstract
A reversed-phase high performance liquid chromatographic method was developed to simultaneously determine two major biflavones, amentoflavone and hinokiflavone, in Trogopterus Feces. The chromatographic separation was achieved with a linear gradient of acetonitrile in 1% acetic acid at a flow rate of 1.0 mL/min using an Alltima C-18 column (250 x 4.6 mm, 5.0 μm). Detection was carried out using a photodiode array detector at 330 nm. The calibration curves of all analytes showed good linearity over the investigated ranges (R 2 > 0.999). Repeatability was evaluated by intra-day and inter-day assays and RSD values were less than 2.40%. The recoveries were 103.5% and 97.3%. This developed method was simple and accurate. The results suggested that the HPLC method could be used to evaluate the quality of Trogopterus Feces from different producing areas.
Recommended Citation
Yang, N.-Y.; Tao, W.-W.; and Duan, J.-A.
(2009)
"Determination of biflavones from trogopterus feces by HPLC,"
Journal of Food and Drug Analysis: Vol. 17
:
Iss.
5
, Article 5.
Available at: https://doi.org/10.38212/2224-6614.2590
