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Abstract

The purpose of this study was to develop a rapid and specific PCR system to detect Shigella spp in samples. A set of primers specific for the virulence gene (VirF) of virulent Shigella spp. and enteroinvasive Escherichia coli (EIEC) produced specific amplicons of expected sizes of 443 bp and 331 bp by using VirF2-VirF3 and VirF3-VirF4 primer sets, respectively. These primers were then used for the detection of food with 101 cells/g inoculation of Shigella spp., followed by Shigella broth incubation. The presence of this pathogen in artificial contaminated foods was detected. Finally, we used this method for the detection of 100 samples, and found that none of Shigella spp. and EIEC bacterial strains was detected in all tested samples by PCR method and the Bacteriological Analytical Manu (BAM) method. The results indicate that the described PCR method has the advantage of a rapid, sensitivity and specificity for use in natural samples.

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