Optimization conditions for extracting polysaccharide from Angelica sinensis and its antioxidant activities

Authors

S. Tian
C. Hao
G. Xu
J. Yang
R. Sun

Abstract

In this study, polysaccharides from Angelica sinensis were extracted using the ultrasound-assisted extraction method. Based on the results of single factor experiments and orthogonal tests, three independent variables—water/raw material ratio, ultrasound time, and ultrasound power—were selected for investigation. Then, we used response surface methodology to optimize the extraction conditions. The experimental data were fitted to a quadratic equation using multiple regression analysis, and the optimal conditions were as follows: water/raw material ratio, 43.31 mL/g; ultrasonic time, 28.06 minutes; power, 396.83 W. Under such conditions, the polysaccharide yield was 21.89 ± 0.21%, which was well matched with the predicted yield. In vitro assays, scavenging activity of superoxide anion radicals, hydroxyl radicals, and 2,2-diphenyl-1-picry-hydrazyl radical showed that polysaccharides had certain antioxidant activities and that hydroxyl radicals have a remarkable scavenging capability. Therefore, these studies provide reference for further research and rational development of A. sinensis polysaccharide. © 2016

Abstract Image

ScienceDirect Link

10.1016/j.jfda.2016.08.012

Recommended Citation

Tian, S.; Hao, C.; Xu, G.; Yang, J.; and Sun, R. (2017) "Optimization conditions for extracting polysaccharide from Angelica sinensis and its antioxidant activities," Journal of Food and Drug Analysis: Vol. 25 : Iss. 4 , Article 27.
Available at: https://doi.org/10.1016/j.jfda.2016.08.012

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This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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