Abstract
The serious cytotoxicity of tyramine attracted marked attention as it induced necrosis of human intestinal cells. This paper presented a novel and facile high performance thin-layer chromatography (HPTLC) method tailored for screening tyramine in cheese. Separation was performed on glass backed silica gel plates, using methanol/ethyl acetate/ammonia (6/4/1 v/v/v) as the mobile phase. Special efforts were focused on optimizing conditions (substrate preparation, laser wavelength, salt types and concentrations) of surface enhanced Raman spectroscopy (SERS) measurements directly on plates after derivatization, which enabled molecule-specific identification of targeted bands. In parallel, fluorescent densitometry (FLD) scanning at 380
ScienceDirect Link
Recommended Citation
Wang, L.; Xu, X.-M.; Chen, Y.-S.; Ren, J.; and Liu, Y.-T.
(2018)
"HPTLC-FLD-SERS as a facile and reliable screening tool: Exemplarily shown with tyramine in cheese,"
Journal of Food and Drug Analysis: Vol. 26
:
Iss.
2
, Article 45.
Available at: https://doi.org/10.1016/j.jfda.2017.07.007
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Fulltext URL
https://www.sciencedirect.com/science/article/pii/S1021949817301448/pdfft?md5=100de8fb9131ec32fc9a6d99ae2aaa7d&pid=1-s2.0-S1021949817301448-main.pdf
Included in
Food Science Commons, Medicinal Chemistry and Pharmaceutics Commons, Pharmacology Commons, Toxicology Commons
Abstract Image