Identification of bacteria in juice/lettuce using magnetic nanoparticles and selected reaction monitoring mass spectrometry

Authors

C.-T. Chen
J.-W. Yu
Y.-P. Ho

Abstract

Ensuring food safety requires a rapid and reliable method for detecting food-borne pathogens. Mass spectrometry has been demonstrated as a powerful tool to classify pure bacterial species. However, matrix interference from food backgrounds may lead to false results because of the suppression of microbial signals. It is useful to develop a method for bacterial enrichment and marker identification in food samples. Magnetic zirconia nanoparticles were used to concentrate spiked microorganisms from apple juice/lettuce under specific conditions (pH 4.5). Bacterial identification was achieved using nanoLC–MS. Selected reaction monitoring of bacteria-related peptides was applied for the first time to identify bacteria including Staphylococcus aureus and Escherichia coli. This study presents an accurate means for bacterial identification in food matrixes using MS. The analysis time is less than 90 min and the minimum concentration of E. coli detected was 5 × 10 3 CFU/mL. The interaction between bacteria and the magnetic nanoparticles was electrostatic and nonspecific, in contrast to immunoassays which require specific antibodies. The targeted peptide analysis focuses on the bacterial markers, thus significantly simplifying the analysis and leading to an accurate identification of bacteria. © 2018

Abstract Image

ScienceDirect Link

10.1016/j.jfda.2018.09.006

Recommended Citation

Chen, C.-T.; Yu, J.-W.; and Ho, Y.-P. (2019) "Identification of bacteria in juice/lettuce using magnetic nanoparticles and selected reaction monitoring mass spectrometry," Journal of Food and Drug Analysis: Vol. 27 : Iss. 2 , Article 21.
Available at: https://doi.org/10.1016/j.jfda.2018.09.006

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This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Fulltext URL

https://www.sciencedirect.com/science/article/pii/S102194981830156X/pdfft?md5=f29d498c105772e557db3aaf5f0deae5&pid=1-s2.0-S102194981830156X-main.pdf