Preparation, evaluation and metabolites study in rats of novel Isoginkgetin-loaded TPGS/soluplus mixed nanomicelles

Authors

Xue Feng, Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University,ChinaFollow
Yu-Ting Chen, Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University,ChinaFollow
Lu-Ya LiFollow
Yu-Peng Sun, Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University,ChinaFollow
Hai-Rong Wang, Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University,ChinaFollow
Lan-Tong Zhang, Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University,ChinaFollow

Keywords

Isoginkgetin, Nanomicelle, Cytotoxicity, Cellular uptake, Metabolite

Abstract

At present, cancer is one of the most lethal diseases in the world, and researchers are committed to developing effective anticancer drugs. Isoginkgetin (IGG) is a kind of biflavone with the potential to treat cancer due to the features of altering the cell cycle and inhibiting tumor cell infiltration. However, its solubility, absorbability and bioavailability are poor, so in this study, IGG was prepared into mixed nanomicelles and evaluated in vitro and in vivo. After condition optimization, IGG-loaded TPGS/soluplus mixed nanomicelles with particle size of 62.34 ± 1.10 nm, entrapment efficiency of 96.92 ± 0.66% and drug loading of 2.42 ± 0.02% were successfully prepared. The physicochemical properties of the nanomicelles were stable within 60 days, and the cytotoxicity of the nanomicelles was significantly higher than that of IGG. The metabolism results showed that 32 kinds of metabolites of IGG and 21 kinds of IGG-loaded nanomicelles were detected. The metabolites of IGG can only be detected in feces of rats, while the metabolites of IGG-loaded nanomicelles can be detected in plasma, bile, urine and feces. All these indicated that after prepared into nanomicelles, the stability, solubility, cytotoxicity and bioavailability of IGG were increased significantly, which provided a new choice for the development of new drugs.

Abstract Image

Recommended Citation

Feng, Xue; Chen, Yu-Ting; Li, Lu-Ya; Sun, Yu-Peng; Wang, Hai-Rong; and Zhang, Lan-Tong (2020) "Preparation, evaluation and metabolites study in rats of novel Isoginkgetin-loaded TPGS/soluplus mixed nanomicelles," Journal of Food and Drug Analysis: Vol. 28 : Iss. 2 , Article 12.
Available at: https://doi.org/10.38212/2224-6614.1065

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Publisher Note

Due to printer error, this article was previously made available with incorrect page numbers. The article has been repaginated and the article is now available with the correct page numbers for volume 28, issue 2 309-321.