Analysis of flavor and other metabolites in lemon juice (Citrus limon) from Huanglongbing-affected trees grafted on different rootstocks

Authors

Laura Reuss, Citrus Research and Education Center, Food Science and Human Nutrition, University of Florida, Lake Alfred, Florida, USAFollow
Shi Feng, Citrus Research and Education Center, Food Science and Human Nutrition, University of Florida, Lake Alfred, Florida, USAFollow
Wei-Lun Hung, Citrus Research and Education Center, Food Science and Human Nutrition, University of Florida, Lake Alfred, Florida, USAFollow
Qibin Yu, Citrus Research and Education Center, Horticultural Science, University of Florida, Lake Alfred, Florida, USAFollow
Frederick G. Gmitter Jr, Citrus Research and Education Center, Horticultural Science, University of Florida, Lake Alfred, Florida, USAFollow
Yu Wang, Citrus Research and Education Center, Food Science and Human Nutrition, University of Florida, Lake Alfred, Florida, USAFollow

Keywords

Huanglongbing, Aroma-active, Taste-active, Metabolites, Rootstocks

Abstract

Flavor perception integrates sensory input from chemical receptors triggered by taste- and aroma-active metabolites to contribute to food flavor quality. Many factors alter flavor quality. Disease affects aroma and flavonoid constituents, causing off-flavors in plants. Huanglongbing (HLB) disease negatively affects citrus, although lemons are more tolerant. Lemon juice quality of HLB-affected fruits is not well studied. Lemon juice aroma-active compounds were profiled in this study using gas chromatography/mass spectrometry-olfactometry, and other metabolites contributing to overall flavor quality were investigated. Lemon juice from different rootstocks was discriminated using the metabolic profile. Flavor and other lemon juice metabolites also distinguished symptomatic from asymptomatic trees. Pathway enrichment analysis demonstrated that biosynthesis pathways of the Phosphotransferase system (PTS), and Starch and sucrose metabolism involving fructose, glucose and sucrose, were highly influenced by HLB status. This study provides the first comprehensive view of lemon juice metabolites, including alterations resulting from rootstock differences or disease severity.

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Recommended Citation

Reuss, Laura; Feng, Shi; Hung, Wei-Lun; Yu, Qibin; Gmitter, Frederick G. Jr; and Wang, Yu (2020) "Analysis of flavor and other metabolites in lemon juice (Citrus limon) from Huanglongbing-affected trees grafted on different rootstocks," Journal of Food and Drug Analysis: Vol. 28 : Iss. 2 , Article 7.
Available at: https://doi.org/10.38212/2224-6614.1060

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This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Publisher Note

Due to printer error, this article was previously made available with incorrect page numbers. The article has been repaginated and the article is now available with the correct page numbers for volume 28, issue 2 261-272.