Quantification of Adenosine Mono-, Di- and Triphosphate from Royal Jelly using Liquid Chromatography - Tandem Mass Spectrometry

Wan-Rou Liao, National Taiwan Normal University, Taipei, Taiwan
Jen-Pang Huang, MSonline Scientific Co., Ltd., Taipei, Taiwan
Sung-Fang Chen, National Taiwan Normal University, Taipei, TaiwanFollow

Keywords

dSPE, HPLC-MS/MS, Nucleotide, Royal jelly, SPE

Abstract

Nucleotides are composed of nitrogen bases, ribose units and phosphate groups. Adenine (Ade), adenosine monophosphate (AMP), adenosine diphosphate (ADP) and adenosine triphosphate (ATP) all play important roles in physiological metabolism. Royal jelly, a secretion produced by worker bees, contains a variety of natural ingredients and several studies have shown that royal jelly can serve as a source of nutrition for humans. In this study, a rapid and effective LC/MS method coupled with pre-processing methods was developed and validated for the accurate quantification of Ade, AMP, ADP and ATP in royal jelly. To achieve the best extraction efficiency, two pretreatment methods, namely, solidphase extraction (SPE) and dispersive solid-phase extraction (dSPE), were developed and investigated. Silica-based cyanopropyl (CN) liquid chromatography was employed using pH programming with a quaternary mobile phase system for the analyses. The total LC/MS run time was less than 12 min with a constant flow rate of 0.25 mL/min. The linear range were 2.5-1000 ng/mL with a correlation coefficient r ¼ 0.9995. The limit of detection (LOD) of Ade, AMP, ADP and ATP was 1, 1, 2.5 and 5 ng/mL; the limit of quantitation (LOQ) was 2.5, 2.5, 5 and 10 ng/mL, respectively. Precision (RSD%

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Recommended Citation

Liao, Wan-Rou; Huang, Jen-Pang; and Chen, Sung-Fang (2020) "Quantification of Adenosine Mono-, Di- and Triphosphate from Royal Jelly using Liquid Chromatography - Tandem Mass Spectrometry," Journal of Food and Drug Analysis: Vol. 28 : Iss. 3 , Article 2.
Available at: https://doi.org/10.38212/2224-6614.1007