Development of the Species-Specific Multiplex PCR and DNA Sequencing Methods for Rapid Authentication of Isatidis Folium and its Adulterants

Authors

Yung-Chuan Hsieh, Food and Drug Administration, MOHW, Taipei, Taiwan
Ming-Sian Wu, Food and Drug Administration, MOHW, Taipei, Taiwan
Hui-Chun Lee, Food and Drug Administration, MOHW, Taipei, Taiwan
Chia-Yun Hsieh, Food and Drug Administration, MOHW, Taipei, Taiwan
Shih-Shan Huang, Food and Drug Administration, MOHW, Taipei, Taiwan
Chia-Fen Tsai, Food and Drug Administration, MOHW, Kaohsiung, Taiwan
Ya-Tze Lin, Food and Drug Administration, MOHW, Taipei, TaiwanFollow
Mei-Chih Lin, Food and Drug Administration, MOHW, Taipei, Taiwan
Su-Hsiang Tseng, Food and Drug Administration, MOHW, Taipei, Taiwan
Der-Yuan Wang, Food and Drug Administration, MOHW, Taipei, Taiwan

Keywords

DNA sequencing, ISATIDIS FOLIUM, Multiplex PCR, trnH-psbA intergenic spacer

Abstract

Isatis indigotica Fort. (family Cruciferae), is an herb widely used in traditional herbal medicine and its dried leave was named “ISATIDIS FOLIUM”. Baphicacanthus cusia (Ness) Bremek. and Polygonum tinctorium Ait. are commonly misused as ISATIDIS FOLIUM in Chinese Medicine pharmacy. For the purpose of being not misused, specific primers based on the sequence difference of chloroplast trnH-psbA intergenic spacer were designed and Multiplex polymerase chain reaction method (Multiplex PCR) was developed. In this study, 29 original herbal materials were analyzed and our results show that DNA size after Multiplex PCR was able to distinguish variations between three herbs. DNA fragments of 464, 297, 170 base pairs (bps) were represented for I. indigotica and B. cusia and P. tinctorium, respectively. In conclusion, our investigations demonstrate that molecular identification method provides more accurate results for medicinal plants detection and good quality control of ISATIDIS FOLIUM.

Abstract Image

Recommended Citation

Hsieh, Yung-Chuan; Wu, Ming-Sian; Lee, Hui-Chun; Hsieh, Chia-Yun; Huang, Shih-Shan; Tsai, Chia-Fen; Lin, Ya-Tze; Lin, Mei-Chih; Tseng, Su-Hsiang; and Wang, Der-Yuan (2021) "Development of the Species-Specific Multiplex PCR and DNA Sequencing Methods for Rapid Authentication of Isatidis Folium and its Adulterants," Journal of Food and Drug Analysis: Vol. 29 : Iss. 2 , Article 9.
Available at: https://doi.org/10.38212/2224-6614.3354

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