Induction of mitochondrial-dependent apoptosis by essential oil of Toona sinensis root through Akt, mTOR and NF-κB signalling pathways in human renal cell carcinoma cells

Authors

Yu-Chi Chen, Department of Urology, E-Da Cancer Hospital, Kaohsiung, TaiwanFollow
Chiao-Ling Hsieh, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, TaiwanFollow
Bu-Miin Huang, Department of Anatomy, College of Medicine, National Cheng Kung University, Tainan, TaiwanFollow
YungChia Chen, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, TaiwanFollow

Keywords

Apoptosis, Essential oil, Renal cell carcinoma, Signalling pathway, Toona sinensis

Abstract

Natural products have long been considered as a kind of complementary medicine. In this study, we investigate the apoptotic effect of essential oils of Toona sinensis roots (TSR) on human clear cell renal cell carcinomas (ccRCC). The sesquiterpene content of TSR essential oil was determined via GC/MS analysis. TSR decreased ccRCC cell viabilities, inducing ROS generation and reduction of the mitochondrial membrane potential. Moreover, TSR inhibited Bcl-2 and Hsp90 expression but increased PARP-1 cleavage and cytochrome c release. Akt, mTOR and NF-kB phosphorylation and HIF-a expression were all inhibited, which likely contributed to the anti-proliferative and anti-adhesive effects of TSR.

Abstract Image

Recommended Citation

Chen, Yu-Chi; Hsieh, Chiao-Ling; Huang, Bu-Miin; and Chen, YungChia (2021) "Induction of mitochondrial-dependent apoptosis by essential oil of Toona sinensis root through Akt, mTOR and NF-κB signalling pathways in human renal cell carcinoma cells," Journal of Food and Drug Analysis: Vol. 29 : Iss. 3 , Article 4.
Available at: https://doi.org/10.38212/2224-6614.3367

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