Mesonosides A-H, primeverose derivatives from Mesona procumbens suppress adipogenesis by downregulating PPARγ and C/EBPa in 3T3-L1 cells

Hung-Tse Huang, Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei, Taiwan
Chia-Ching Liaw, Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei, Taiwan
Chun-Tang Chiou, Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei, Taiwan
Kung-Ta Lee, Department of Biochemical Science and Technology, National Taiwan University, Taipei, TaiwanFollow
Yao-Haur Kuo, Department of Biochemical Science and Technology, National Taiwan University, Taipei, TaiwanFollow

Keywords

Anti-adipogenic, Lipid accumulation, Mesona procumbens, Mesonoside, PPARγ

Abstract

Obesity is becoming a worldwide epidemic, especially in industrialized countries. We hereby report a methanolic extract of Mesona procumbens (known as Hsian-tsao in Taiwan) significantly inhibits lipid accumulation in 3T3-L1 adipocytes, and eight new primeverose derivatives, mesonosides A-H (1-8), were isolated from the methanolic extract of M. procumbens. Structural elucidation of 1-8 was established by spectroscopic methods, especially 2D NMR techniques (¹H-¹H COSY, HSQC, HMBC, and NOESY) and HRESIMS. Anti-obesity evaluation revealed that isolates 1-5, 7, and 8 showed inhibitory effects on lipid accumulation and protein levels of adipogenic transcription factor, PPARγ and C/EBPa in 3T3-L1 cells. Our study suggests that M. procumbens extract including new primeverose isolates may bepotentially used as a natural source to ameliorate fat accumulation and even obesity.

Abstract Image

Recommended Citation

Huang, Hung-Tse; Liaw, Chia-Ching; Chiou, Chun-Tang; Lee, Kung-Ta; and Kuo, Yao-Haur (2021) "Mesonosides A-H, primeverose derivatives from Mesona procumbens suppress adipogenesis by downregulating PPARγ and C/EBPa in 3T3-L1 cells," Journal of Food and Drug Analysis: Vol. 29 : Iss. 3 , Article 5.
Available at: https://doi.org/10.38212/2224-6614.3365