Lucigenin-pyrogallol chemiluminescence for the multiple detection of pyrogallol, cobalt ion, and tyrosinase

Islam M. Mostafa, State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,Changchun, Jilin, PR China
Muhammad Rehan Hasan Shah Gilani, State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,Changchun, Jilin, PR China
Yequan Chen, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, China
Baohua Lou, University of Science and Technology of China, Hefei, PR ChinaFollow
Jianping Li, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, ChinaFollow
Guobao Xu, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, ChinaFollow

Keywords

Chemiluminescence, Cobalt, Lucigenin, Pyrogallol, Tyrosinase

Abstract

Developing new, cheap, sensitive and selective chemiluminescence (CL) systems with multiple detection properties is still a big challenge for biological and environmental applications. Here, we report a new CL system having multiple detection applications (environmental and biological). The developed lucigenin-pyrogallol system gave an enhancement (190 times) over the conventional lucigenin-H₂O₂ CL system. Cobalt ion and tyrosinase can inhibit lucigenin-PG CL intensity. Based on these phenomena, we have developed new CL methods for the detection of pyrogallol (LOD = 0.94 μM), lucigenin (LOD = 0.42 μM), cobalt (LOD = 68 μM), and tyrosinase (LOD = 1.8 μg/mL). Furthermore, the developed system gave excellent recoveries in real samples within the range of 93.59-103.12% for the assay of pyrogallol, Co²⁺ and tyrosinase in coffee, lake water, and human plasma, respectively.

Abstract Image

Recommended Citation

Mostafa, Islam M.; Rehan Hasan Shah Gilani, Muhammad; Chen, Yequan; Lou, Baohua; Li, Jianping; and Xu, Guobao (2021) "Lucigenin-pyrogallol chemiluminescence for the multiple detection of pyrogallol, cobalt ion, and tyrosinase," Journal of Food and Drug Analysis: Vol. 29 : Iss. 3 , Article 9.
Available at: https://doi.org/10.38212/2224-6614.3361