Anti-lymphangiogenic diterpenes from the bark of Calocedrus macrolepis var. formosana

Tzong-Huei Lee, Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan
Chin-Lin Hsieh, Department of Chemistry, National Taiwan University, Taipei, Taiwan
Ho-Cheng Wu, Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan
Shih-Wei Wang, Institute of Biomedical Sciences, MacKay Medical College, New Taipei City, Taiwan
Chen-Lin Yu, Institute of Biomedical Sciences, MacKay Medical College, New Taipei City, Taiwan
George Hsiao, Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan
Ming-Jen Cheng, Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu, Taiwan
Wen-Tsong Hsieh, Department of Pharmacology, China Medical University, Taichung, Taiwan
Yueh-Hsiung Kuo, Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, TaiwanFollow

Keywords

Anti-lymphangiogenesis; Calocedrus macrolepis; Diterpenes

Abstract

Eight new diterpenes, 6α,7β-dihydroxyferruginol (1), 6α,7α-dihydroxyferruginol (2), 6α-hydroxyhinokiol (3), 4α-hydroxy-7-oxo-18-norabieta-8,11,13-trien-4α-ol (4a), 15,16-dehydrosugiol (5), 7-methoxy-6,7-secoabieta-8,11,13-triene-6,12-diol (6), 7α-acetoxyabieta-8,12-diene-11,14-dione (7), 7α-butyloxyethyloxyabieta-8,12-diene-11,14-dione (8), along with four known compounds, 6,7-dehydroferruginol (9), 12-hydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial (10), 7α-11-dihydroxy-12-methoxy-8,11,13-abietatriene (11), and 11,14-dihydroxy-8,11,13-abietatrien-7-one (12) were successfully isolated from the bark of Calocedrus macrolepis var. formosana. The structures of all isolates were elucidated by physical data (appearance, UV, IR, optical rotation) and spectroscopic data (1D, 2D NMR, and HREIMS). Compounds 9, 10, 11, and 12 showed promising growth-inhibitory effect on human lymphatic endothelial cells (LECs). Among these compounds, compound 10 exerted the most potent anti-lymphangiogenesis property by suppressing cell growth and tube formation of LECs. In conclusion, the results revealed the anti-lymphangiogenic potentials of Formosan C. macrolepis var. formosana.

Keywords: Anti-lymphangiogenesis, Calocedrus macrolepis var.formosana, Diter-penes

Abstract Image

Recommended Citation

Lee, Tzong-Huei; Hsieh, Chin-Lin; Wu, Ho-Cheng; Wang, Shih-Wei; Yu, Chen-Lin; Hsiao, George; Cheng, Ming-Jen; Hsieh, Wen-Tsong; and Kuo, Yueh-Hsiung (2021) "Anti-lymphangiogenic diterpenes from the bark of Calocedrus macrolepis var. formosana," Journal of Food and Drug Analysis: Vol. 29 : Iss. 4 , Article 5.
Available at: https://doi.org/10.38212/2224-6614.3375