Direct immersion solid-phase microextraction combined with ambient ionization tandem mass spectrometry to rapidly distinguish pesticides in serum for emergency diagnostics

Hung Su, Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan
Hsin-Hua Lin, Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, Taiwan
Lin-Jhen Su, Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, Taiwan
Chia-Chen Lin, Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, Taiwan
Zong-Han Jiang, Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, Taiwan
Shiang-Jin Chen, Department of Emergency Medicine, Kaohsiung Medical University Hospital, Kaohsiung Taiwan
Jentaie Shiea, Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan
Chi-Wei Lee, Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, TaiwanFollow

Keywords

Carbamate, Emergency care, Organophosphate, Pesticides, Solid-phase microextraction, Thermal desorption-electrospray ionization tandem mass spectrometry

Abstract

Despite the fact that carbamates and organophosphates cause acute poisoning via different mechanisms and require disparate management, they are indistinguishable by current clinical assays. Herein, direct immersion solid-phase microextraction (DI-SPME) plus thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was developed to discern them. Both pesticides spiked in human serum were extracted by SPME and analyzed by TD-ESI/MS/MS. This is a promising emergency care platform as rapid analyses could be done in tiny sample volumes with satisfactory recovery (89.46%–116.32%), precision (covariance < 20%), sensitivity (LOD < 0.1 µg/mL), turnaround time (< 5 minutes), and linearity (R² = 0.9827–0.9992) within 0.1–100 µg/mL.

Abstract Image

Recommended Citation

Su, Hung; Lin, Hsin-Hua; Su, Lin-Jhen; Lin, Chia-Chen; Jiang, Zong-Han; Chen, Shiang-Jin; Shiea, Jentaie; and Lee, Chi-Wei (2022) "Direct immersion solid-phase microextraction combined with ambient ionization tandem mass spectrometry to rapidly distinguish pesticides in serum for emergency diagnostics," Journal of Food and Drug Analysis: Vol. 30 : Iss. 1 , Article 3.
Available at: https://doi.org/10.38212/2224-6614.3399