A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma

Cheng-Wei Cheng, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC
Wen-Han Feng, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC
Wei-Hua Tang, Taipei Veterans General Hospital Yuli Branch, Hualian, Taiwan, ROC
Hwang-Shang Kou, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC
Chun-Chi Wang, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROCFollow
Shou-Mei Wu, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROCFollow

Keywords

Anticoagulant, Dabigatran, Dabigatran acyl-beta-d-glucuronide, FASI-sweeping-AFMC, Human plasma

Abstract

A three-step stacking capillary electrophoresis (CE) composed of field-amplified sample injection, sweeping, and analyte focusing by micellar collapse (FASI-sweeping-AFMC) was developed to determine dabigatran (D) and its major active metabolite, dabigatran acyl-beta-d-glucuronide (DAG), in human plasma. After optimization and validation, this novel approach was further applied to monitor 5 real samples, and the 25.2 to 186.8 ng mL^-1 D could be observed among those. Based on these results, the novel CE stacking strategy was successfully applied for the analysis of D and DAG in human plasma and could be served as a tool for clinical assays.

Abstract Image

Recommended Citation

Cheng, Cheng-Wei; Feng, Wen-Han; Tang, Wei-Hua; Kou, Hwang-Shang; Wang, Chun-Chi; and Wu, Shou-Mei (2022) "A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma," Journal of Food and Drug Analysis: Vol. 30 : Iss. 1 , Article 8.
Available at: https://doi.org/10.38212/2224-6614.3391