Exploring the relationship between metabolite composition and the cold/hot properties ascribed in traditional Chinese medicine by mass spectral molecular networking–a pilot study

Authors

Chun-Han Su, Agricultural Biotechnology Research Center, Academia Sinica, Taiwan
Yu-Chieh Cheng, Agricultural Biotechnology Research Center, Academia Sinica, Taiwan
Kuei-Hung Lai, PhD Program in Clinical Drug Development of Herbal Medicine, College of Pharmacy, Taipei Medical University, Taiwan
Yu-Chia Chang, Research Center for Chinese Herbal Medicine, Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taiwan
Chi-Hui Sun, Agricultural Biotechnology Research Center, Academia Sinica, Taiwan
Po-Wen Tu, Agricultural Biotechnology Research Center, Academia Sinica, Taiwan
Ching-Chih Lin, Agricultural Biotechnology Research Center, Academia Sinica, Taiwan
Tsong-Long Hwang, Research Center for Chinese Herbal Medicine, Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taiwan
Yu-Liang Yang, Agricultural Biotechnology Research Center, Academia Sinica, TaiwanFollow

Keywords

LC-MS/MS; Medicinal property; Metabolome mining; Molecular networking; Traditional Chinese medicine

Abstract

Traditional Chinese medicine (TCM) has been applied to improve human health for millennia. In the TCM system, “medicinal property” (yao xing; hot and cold properties) is a core concept used to describe the influences of medicinal materials on human physiological conditions, and metabolites are believed to be one of the major ingredients of TCMs that affect their medicinal property. However, due to a lack of comprehensive analyses of TCM metabolomes, information about the relationships between TCM metabolite composition and medicinal property remains limited. In this pilot study, a mass spectral molecular networking-based platform was established and applied to systematically profile the metabolome of 24 TCMs with various medicinal properties. The molecular networks were built based on the liquid chromatography-tandem mass spectrometry (LC-MS/MS) data from 50% EtOH extracts of 24 TCMs. The results showed that various classes of metabolites were clustered in the molecular networks, and the potential medicinal property-associated molecular families were filtered by screening the medicinal property and the diversity of TCM sources. For example, some specific types of flavonoids were identified in the representative cold-property (han xing) molecular families. In contrast, due to the limited sample size, the representative and universal hot-property (re xing) molecular family has not been well revealed. In summary, this study provides methodology and information on the potential relationships between the metabolite composition and the concept of medicinal property in TCM. Furthermore, the results can serve as a foundation for mass spectral molecular networking-based analysis of TCM metabolomes, facilitating TCM research and development.

Abstract Image

Recommended Citation

Su, Chun-Han; Cheng, Yu-Chieh; Lai, Kuei-Hung; Chang, Yu-Chia; Sun, Chi-Hui; Tu, Po-Wen; Lin, Ching-Chih; Hwang, Tsong-Long; and Yang, Yu-Liang (2022) "Exploring the relationship between metabolite composition and the cold/hot properties ascribed in traditional Chinese medicine by mass spectral molecular networking–a pilot study," Journal of Food and Drug Analysis: Vol. 30 : Iss. 3 , Article 6.
Available at: https://doi.org/10.38212/2224-6614.3425

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