Chemical constituents from a marine medicinal brown alga-derived Xylaria acuta SC1019

Hsiao-Yang Hsi, Institute of Fisheries Science, National Taiwan University, Taipei 106, Taiwan
Shih-Wei Wang, Institute of Biomedical Sciences, MacKay Medical College, New Taipei City 252, Taiwan
George Hsiao, Graduate Institute of Medical Sciences and Department of Pharmacology, School of Medicine, College of Medicine, Taipei 110, Taiwan
Li-Kwan Chang, Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei 106, Taiwan
Yuan-Chung Cheng, Department of Chemistry and Center for Quantum Science and Engineering, National Taiwan University, Taipei 106, Taiwan
Shu-Jung Huang, Institute of Fisheries Science, National Taiwan University, Taipei 106, Taiwan
Yi-Shan Lu, Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei 106, Taiwan
Tzong-Huei Lee, Institute of Fisheries Science, National Taiwan University, Taipei 106, TaiwanFollow

Keywords

Anti-angiogenesis, Anti-inflammation, Xylaria acuta, Xylariaceae

Abstract

In this study, a marine medicinal brown alga Sargassum cristaefolium-derived fungal strain Xylaria acuta SC1019 was isolated and identified. Column chromatography of the extracts from liquid- and solid-fermented products of the fungal strain was carried out, and led to the isolation of twenty-one compounds. Their structures were characterized by spectroscopic analysis, and the absolute configurations were further established by single X-ray diffraction analysis or modified Mosher’s method as nine previously undescribed compounds, namely xylarilactones A–C (1–3), ent-gedebic acid 8-O-α-D-glucopyranoside (4), 5R-hydroxylmethylmellein 11-O-α-D-glucopyranoside (5), ent-hymatoxin E 16-O-α-D-mannopyranoside (6), 19,20-epoxycytochalasin S (7), 19,20-epoxycytochalasin T (8), and (2R)-butylitaconic acid (9), along with twelve known compounds 10–21. All the isolates were subjected to anti-inflammatory and anti-angiogenic assays. Compounds 1, 5, 7, 10, and 17 showed moderate nitric oxide production inhibitory activities in lipopolysaccharide-activated BV-2 microglial cells with IC₅₀ values of 19.55 ± 0.35, 16.10 ± 0.57, 15.20 ± 0.87, 11.76 ± 0.49, and 11.30 ± 0.32 μM, respectively, as compared to curcumin (IC₅₀ = 2.69 ± 0.34 μM) without any significant cytotoxicity. Compounds 7, 8, and 21 displayed potent anti-angiogenic activities by suppressing the growth of human endothelial progenitor cells with IC₅₀ values of 0.44 ± 0.01, 0.47 ± 0.03, and 0.53 ± 0.01 μM, respectively, as compared to sorafenib (IC₅₀= 5.50 ± 1.50 μM).

Abstract Image

Recommended Citation

Hsi, Hsiao-Yang; Wang, Shih-Wei; Hsiao, George; Chang, Li-Kwan; Cheng, Yuan-Chung; Huang, Shu-Jung; Lu, Yi-Shan; and Lee, Tzong-Huei (2024) "Chemical constituents from a marine medicinal brown alga-derived Xylaria acuta SC1019," Journal of Food and Drug Analysis: Vol. 32 : Iss. 2 , Article 3.
Available at: https://doi.org/10.38212/2224-6614.3501