Alanine supplementation enhancing cordycepin production in Cordyceps militaris via upregulation of Cns2 and Cns3 genes expression levels

Authors

Wenbin Yu, Jiangxi Key Laboratory of Natural Microbial Medicine Research, College of Life Sciences, Jiangxi Science & Technology normal university, Nanchang 330013, Jiangxi, China
Zeying Zhao, Jiangxi Key Laboratory of Natural Microbial Medicine Research, College of Life Sciences, Jiangxi Science & Technology normal university, Nanchang 330013, Jiangxi, China
Bin Zeng, Jiangxi Key Laboratory of Natural Microbial Medicine Research, College of Life Sciences, Jiangxi Science & Technology normal university, Nanchang 330013, Jiangxi, China; College of Pharmacy, Shenzhen Technology University, Shenzhen 518000, Guangdong, China
Yayi Tu, Jiangxi Key Laboratory of Natural Microbial Medicine Research, College of Life Sciences, Jiangxi Science & Technology normal university, Nanchang 330013, Jiangxi, ChinaFollow
Bin He, Jiangxi Key Laboratory of Natural Microbial Medicine Research, College of Life Sciences, Jiangxi Science & Technology normal university, Nanchang 330013, Jiangxi, ChinaFollow

Keywords

Alanine, Cordyceps militaris, Cordycepin, Secondary metabolites, Transcriptome

Abstract

Cordycepin, a key bioactive compound produced by Cordyceps militaris, faces the challenge of low productivity forcommercial use. In this study, alanine supplementation in Cordyceps militaris boosted cordycepin production, peakingat 3 mg/g with 12 g/L concentration. Transcriptome analysis revealed 1711 differentially expressed genes, Pathwayanalysis indicates that protein processing in the endoplasmic reticulum was the most affected pathway. In addition, thetranscriptome showed that adenylosuccinate lyase is essential for the synthesis of cordycepin. The modulation of fourgenes (Cns1-4) points to a regulatory mechanism that could increase cordycepin biosynthesis, offering a strategy to overcome low productivity for commercial applications.

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Recommended Citation

Yu, Wenbin; Zhao, Zeying; Zeng, Bin; Tu, Yayi; and He, Bin (2024) "Alanine supplementation enhancing cordycepin production in Cordyceps militaris via upregulation of Cns2 and Cns3 genes expression levels," Journal of Food and Drug Analysis: Vol. 32 : Iss. 4 , Article 16.
Available at: https://doi.org/10.38212/2224-6614.3529

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